Coding

Part:BBa_K523011:Design

Designed by: Sylvia Ispasanie, Mun Ching Lee   Group: iGEM11_Edinburgh   (2011-08-04)


Endoglucanase for BioSandwich


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 141
    Illegal XhoI site found at 503
    Illegal XhoI site found at 752
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 283
    Illegal NgoMIV site found at 1208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 187


Design Notes

For compatibility with BioSandwich, an internal BglII site had to be removed by site-specific mutagenesis.


Source

From BBa_K118023

References